Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Development of a dual-component infection-resistant arterial replacement for small-caliber reconstructions: A proof-of-concept study

doi: 10.3389/fbioe.2023.957458

Figure Lengend Snippet: Mean DNA (A) , sGAG (B) , HYP (C) and dHYP (D) content per dry weight of native PTA and decellularized PTA scaffolds treated with (M2) or without (M1) RNase/DNase and sterilized with either PAA or γ-irradiation. Data was expressed as means ( n = 6); error bars indicate 95% C.I.; MSD: minimum significant difference; indicates significant difference between the native and the decellularized groups. Connectors indicate significant difference between the originator and end arrow group. (E–G) Characteristic en face multiphoton images of the lumen of decellularized PTAs, treated with M1 and sterilized with either PAA (E,F) or γ-irradiation (G) , showing the elastic fiber network of internal elastic lamina (E, G; imaging plane was approximately 20 μm below lumen surface) and the collagen fibers of tunica media (F; imaging plane was about 60 μm below lumen surface). Black arrow heads (►) show elastic fibers. Scale bars indicate 100 μm (E,F) or 50 μm (G) .

Article Snippet: The multiphoton imaging was performed using a CellSurgeon multiphoton microscope (MPM; LLS-ROWIAK).

Techniques: Irradiation, Imaging

Journal: Scientific Reports

Article Title: Real-time visualization and modulation of endocytic dynamics in osteocytes in vivo

doi: 10.1038/s41598-025-05735-1

Figure Lengend Snippet: Graphical abstract of experimental design for imaging intravital C’Dot uptake and clearance in bone with 2-photon microscopy. A) Bone and osteocytes were exposed to fluorescent nanoparticles through a local subcutaneous injection above the third metatarsal (MT3) bone in a mouse hind paw. After C’Dot incubation, the MT3 was surgically isolated and secured for multiphoton imaging. B-D) Example images of C’Dots in the lacuno-canalicular network are shown. E-F) The high resolution of our imaging system enables subcellular localization. G) A short C’Dot incubation time and high frame averaging allows visualization of the canalicular network. H) Images can be analyzed for clearance kinetics of nanoparticle signal (from previous paper ) as well as subcellular distribution of signal. Scale bars = 10 μm.

Article Snippet: Fluorescent signal inside the functionally isolated MT3 was visualized with multiphoton microscopy (MPM) (Bergamo II, Thorlabs) as previously described .

Techniques: Imaging, Microscopy, Injection, Incubation, Isolation